Effect of Mir-299-3p on the biological function of lung adenocarcinoma cell H1299 through targeting PTPRD

The paper aimed to investigate the effects of up-regulation and down-regulation Mir-299-3p on proliferation, migration, invasion apoptosis lung adenocarcinoma cell line H1299, preliminarily explore mechanism regulating biological behavior cells. inhibitor mimics were transfected into transfection efficiency was measured by fluorescence microscopy. successfully cells detected invasion, apoptosis. used down-regulate up-regulate respectively. Western blot employed detect expressions STAT3, p-STAT3, caspase3, Bcl-2, Bax MMP-9 proteins in Small interfering RNA technology applied construct a with low expression PTPRD. WB measure determine whether PTPRD can partially or completely reverse after Mir-299-3p. It also determined effect MMP-9, Cleaved-caspase3, Bcl-2 Down-regulation inhibit proliferation promote apoptosis, p-STAT3 apoptosis-inducing such as protein Bcl-2. worked opposite way. is direct target gene may directly regulate affect